CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Mammalian and yeast as complementary cell models in Programmed Cell Death and Autophagy

Dates & Venue
19 to 30 June
Departament of Biology, Universidade do Minho
Invited Lecturers
Cecília Rodrigues
Faculty of Pharmacy, University of Lisbon, Portugal
Clara Pereira
IBMC, University of Porto
Muriel Priault
CNRS-Université de Bordeaux, France
Nadine Camougrand
CNRS-Université de Bordeaux, France
Stéphen Manon
CNRS-Université de Bordeaux, France
Vítor Costa
IBMC, University of Porto
SAara Alves
IPATIMUP, University of Porto

In-House






PhD and Master Students Laboratory Assistance
Ana Preto
CBMA-DB, University of Minho, Portugal
Susana Chaves
CBMA-DB, University of Minho, Portugal
António Rego
CBMA-DB, University of Minho, Portugal

Cátia Pereira
Joana Guedes
Joana Matos
Flávia Martins

Topics

Regulate cell death processes versus necrosis (basic concepts)

Apoptosis inyeast  mammalian cells

Programmed necrosis

Autophagy in physiology and pathology

Mitophagy: from yeasts to mammalian cells

Study of mitophagy in yeast

The role of mitochondria in apoptosis

Utilization of yeast to study the molecular aspects of the function of Bcl-2 family members

N-terminal acetylation and cell death: modulation of Bax targeting to mitochondria

Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism

Deamidation of proteins, so much more than just a hallmark of aging

Acetic acid/acetate-induced apoptosis in yeast/mammalian cancer cells

Sphingolipid signaling and oxidative stress in yeast cell death and ageing

Regulation of mitochondrial function and cell death by the yeast Ser/Thr protein phosphatase Sit4p

miRNAs as biomarkers and regulators of liver disease

Pratical session

















Scope of the Course
P1 - Cell death markers in Mammalian cell lines - monitoring caspase activation

P2 - Autophagy/Mitophagy protocol in yeast

P3 - Cell death markers in yeast - monitoring release of HMBG1 and plasma membrane integrity

P4 - Cell death markers in yeast - monitoring cytochrome C release

P5 - Cell death markers in cell lines - monitoring Phisphatidyl mserine exposure

P6 - Cell death markers in Mammalian cell lines - Monitoring DNA fragmentation

This post-graduated course is part of the optional curricular assignments of several Master and PhD courses of the Biology Department and has been organized for post-graduation students or researchers who are interested in the in regulatated cell death and autophagy/mitophagy and human diseases associated with their dysfunction. It includes lectures and seminars as well as a hands-on intensive module of lab work. In addition to brief descriptive lectures, the theoretical modules will entail discussions of scientific papers. The hands-on lab module will illustrate different aspects of the theoretical session topics, and will include different techniques to monitor morphological, biochemical and molecular changes associated to cell death processes and autophagy namely, epifluorescence microscopy and flow cytometry. They will be followed by results interpretation, group discussion and final wrap up.

SCHEDULE

 

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